spcas9 ng (Addgene inc)
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Addgene inc
spcas9 ng
Spcas9 Ng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spcas9+ng/SpCas9-NG+(Plasmid+%23138566)/bio_rxiv__64898__2025__12__20__695720-229-1-38
Average 93 stars, based on 27 article reviews
Spcas9 Ng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spcas9+ng/SpCas9-NG+(Plasmid+%23138566)/bio_rxiv__64898__2025__12__20__695720-229-1-38
Average 93 stars, based on 27 article reviews
spcas9 ng - by Bioz Stars,
2026-10
93/100 stars
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Generated:Article Title: Functional Characterization of DNA Repair Gene Variants in Live Cells Enabled Through Precision Genome Editing, Chemical Biology, and Biochemical Tools Article Snippet: .. Base editor plasmids were generated by taking either AncBE4max-P2A-GFP (Addgene plasmid #112100) or ABE7.10max-P2A-GFP (Addgene plasmid #112101) and replacing the C-terminal 300 amino acids of Cas9n with that from Article Title: Precision genome editing and in-cell measurements of oxidative DNA damage repair enable functional and mechanistic characterization of cancer-associated MUTYH variants Article Snippet: .. Base editor (BE) plasmids were generated by taking either AncBE4max-P2A-GFP (Addgene plasmid #112100) or ABE7.10max-P2A-GFP (Addgene plasmid #112101) and replacing the C-terminal 300 amino acids of Cas9n with that from Article Title: Precision genome editing and in-cell measurements of oxidative DNA damage repair enable functional and mechanistic characterization of cancer-associated MUTYH variants. Article Snippet: .. Base editor (BE) plasmids were generated by taking either AncBE4max-P2A-GFP (Addgene plasmid #112100) or ABE7.10max-P2A-GFP (Addgene plasmid #112101) and replacing the C-terminal 300 amino acids of Cas9n with that D ow nloaded from https://academ ic.oup.com /nar/article/53/6/gkaf037/8099200 by guest on 07 April 2025 from Plasmid Preparation:Article Title: Functional Characterization of DNA Repair Gene Variants in Live Cells Enabled Through Precision Genome Editing, Chemical Biology, and Biochemical Tools Article Snippet: .. Base editor plasmids were generated by taking either AncBE4max-P2A-GFP (Addgene plasmid #112100) or ABE7.10max-P2A-GFP (Addgene plasmid #112101) and replacing the C-terminal 300 amino acids of Cas9n with that from Article Title: Precision genome editing and in-cell measurements of oxidative DNA damage repair enable functional and mechanistic characterization of cancer-associated MUTYH variants Article Snippet: .. Base editor (BE) plasmids were generated by taking either AncBE4max-P2A-GFP (Addgene plasmid #112100) or ABE7.10max-P2A-GFP (Addgene plasmid #112101) and replacing the C-terminal 300 amino acids of Cas9n with that from Article Title: Design of prime-editing guide RNAs with deep transfer learning Article Snippet: The pCMV-PE2-P2A-hMLH1dn plasmid was purchased from Addgene (174827). .. The pCMV-NG-PE plasmid was constructed based on Article Title: One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids. Article Snippet: Human codon-optimized base editing constructs were a kind gift from David Liu; pCMV_ABEmax_P2A_GFP (plasmid #112101; Addgene), pCMV_AncBE4max_P2A_GFP (plasmid#112100; Addgene). pCMV_ SpCas9-NG_ABEmax_P2A_GFP, pCMV_SpCas9-NG_AncBE4max_P2A_ GFP and pCMV_SaKKH_AncBE4max_P2A_GFP were constructed by PCR amplification (Q5, NEB) amplifying everything except for SpCas9 using pCMV_ABEmax_P2A_GFP and pCMV_AncBE4max_P2A_GFP. .. Coding sequences for Article Title: Manipulating the 3D organization of the largest synthetic yeast chromosome. Article Snippet: Plugs were loaded onto pulsed field electrophoresis system using 1% agarose gels run in 0.5xTBE buffer (BioRad, CHEF Mapper XA) for chromosome separation. .. Swapping TAG stop codon to TAA by SpCas9-NG in yeast The SpCas9-NG coding sequence was amplified from the Article Title: Precision genome editing and in-cell measurements of oxidative DNA damage repair enable functional and mechanistic characterization of cancer-associated MUTYH variants. Article Snippet: .. Base editor (BE) plasmids were generated by taking either AncBE4max-P2A-GFP (Addgene plasmid #112100) or ABE7.10max-P2A-GFP (Addgene plasmid #112101) and replacing the C-terminal 300 amino acids of Cas9n with that D ow nloaded from https://academ ic.oup.com /nar/article/53/6/gkaf037/8099200 by guest on 07 April 2025 from Cloning:Article Title: Precision genome editing and in-cell measurements of oxidative DNA damage repair enable functional and mechanistic characterization of cancer-associated MUTYH variants Article Snippet: .. Base editor (BE) plasmids were generated by taking either AncBE4max-P2A-GFP (Addgene plasmid #112100) or ABE7.10max-P2A-GFP (Addgene plasmid #112101) and replacing the C-terminal 300 amino acids of Cas9n with that from Article Title: A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets Article Snippet: .. Cloning of xCas9, Article Title: Precision genome editing and in-cell measurements of oxidative DNA damage repair enable functional and mechanistic characterization of cancer-associated MUTYH variants. Article Snippet: .. Base editor (BE) plasmids were generated by taking either AncBE4max-P2A-GFP (Addgene plasmid #112100) or ABE7.10max-P2A-GFP (Addgene plasmid #112101) and replacing the C-terminal 300 amino acids of Cas9n with that D ow nloaded from https://academ ic.oup.com /nar/article/53/6/gkaf037/8099200 by guest on 07 April 2025 from Construct:Article Title: Design of prime-editing guide RNAs with deep transfer learning Article Snippet: The pCMV-PE2-P2A-hMLH1dn plasmid was purchased from Addgene (174827). .. The pCMV-NG-PE plasmid was constructed based on Article Title: A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets Article Snippet: .. Cloning of xCas9, Polymerase Chain Reaction:Article Title: One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids. Article Snippet: Human codon-optimized base editing constructs were a kind gift from David Liu; pCMV_ABEmax_P2A_GFP (plasmid #112101; Addgene), pCMV_AncBE4max_P2A_GFP (plasmid#112100; Addgene). pCMV_ SpCas9-NG_ABEmax_P2A_GFP, pCMV_SpCas9-NG_AncBE4max_P2A_ GFP and pCMV_SaKKH_AncBE4max_P2A_GFP were constructed by PCR amplification (Q5, NEB) amplifying everything except for SpCas9 using pCMV_ABEmax_P2A_GFP and pCMV_AncBE4max_P2A_GFP. .. Coding sequences for Article Title: A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets Article Snippet: .. Cloning of xCas9, Amplification:Article Title: One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids. Article Snippet: Human codon-optimized base editing constructs were a kind gift from David Liu; pCMV_ABEmax_P2A_GFP (plasmid #112101; Addgene), pCMV_AncBE4max_P2A_GFP (plasmid#112100; Addgene). pCMV_ SpCas9-NG_ABEmax_P2A_GFP, pCMV_SpCas9-NG_AncBE4max_P2A_ GFP and pCMV_SaKKH_AncBE4max_P2A_GFP were constructed by PCR amplification (Q5, NEB) amplifying everything except for SpCas9 using pCMV_ABEmax_P2A_GFP and pCMV_AncBE4max_P2A_GFP. .. Coding sequences for Article Title: Manipulating the 3D organization of the largest synthetic yeast chromosome. Article Snippet: Plugs were loaded onto pulsed field electrophoresis system using 1% agarose gels run in 0.5xTBE buffer (BioRad, CHEF Mapper XA) for chromosome separation. .. Swapping TAG stop codon to TAA by SpCas9-NG in yeast The SpCas9-NG coding sequence was amplified from the Sequencing:Article Title: Manipulating the 3D organization of the largest synthetic yeast chromosome. Article Snippet: Plugs were loaded onto pulsed field electrophoresis system using 1% agarose gels run in 0.5xTBE buffer (BioRad, CHEF Mapper XA) for chromosome separation. .. Swapping TAG stop codon to TAA by SpCas9-NG in yeast The SpCas9-NG coding sequence was amplified from the Mutagenesis:Article Title: A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets Article Snippet: .. Cloning of xCas9, |